Coding

Part:BBa_K307106:Design

Designed by: Joo-han Gwak   Group: iGEM10_CBNU-Korea   (2010-10-27)

parB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 161
    Illegal PstI site found at 71
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 161
    Illegal PstI site found at 71
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 161
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 161
    Illegal PstI site found at 71
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 161
    Illegal PstI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

From Vibrio cholerae chromosomeII, using PCR amplified and transformed.

Source

Vibrio cholerae ChromosomeII

References

Yoshiharu yamaichi et al.,"par genens and the pathology of chromosome loss in vibrio cholerae",PNAS,january 9, 2007, vol.104,no.2,630-635

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